Controlled Release of Antigen In Dendritic Cells Using pH-Sensitive Liposome-Polymeric Hybrid Nanoparticles

A nano delivery system that can stay stable during circulation in vivo, as well as enables rapid release of the encapsulated antigen in dendritic cells would be of great value in vaccine development. Here, we present the development of a nano hybrid particle consisting of a pH sensitive lipid shell and a poly (lactic-co-glycolic) acid (PLGA) core. This nanoparticle, with a mean size of 120.1 +/- 8.8 nm and positively charged surface (zeta potential was of 14.2 +/- 1.4 mV), may facilitate its uptake by dendritic cells. The instant disintegration of lipid shell in response to a low pH challenge suggested a rapid release of the enclosed antigen under the acidic environment of endosomes in dendritic cells. The in vitro test showed a faster release of antigen from this particle compared to non-pH sensitive nanoparticles in dendritic cells. The hybrid nanoparticle also exhibited minimal antigen release in human serum at physiological pH, while showing a burst release after pH was reduced to 5.5. All these findings indicate that this hybrid nanoparticle may serve as a promising antigen delivery system for vaccine development.

Category(s):

Drug Delivery

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For Information, Contact:

Rob Whitehead

Senior Licensing Manager

Virginia Tech Intellectual Properties, Inc.

(540) 443-9219

rwhitehead@vtip.org

Inventors:

Chenming (mike) Zhang

Yun Hu