The role of physical forces in disease onset and progression is widely accepted and this knowledge presents an alternative route to investigating disease models. Cells both diseased and non-diseased have differences in force signatures. Measuring forces in real time allows performing diagnostics of cells from patients and also provides a clear path to test drug efficacy. Recently, numerous force measurement techniques have been developed to probe single and multi-cell behavior. While these methods have yielded fundamental insights, they are yet unable to capture the fibrous extra-cellular matrix biophysical interactions, involving parameters of curvature, structural stiffness (N m-1), alignment and hierarchy, which have been shown by us to play key roles in disease and developmental biology. The platform technology presented here quantifies high spatio-temporal cell force modulation (both inside-out and outside-in) with and without the presence of a cytoskeleton altering drug using suspended and aligned fiber networks (nanonets) manufactured using the nonelectrospinning Spinnerer base Tunable Engineering Parameters (STEP) technique. Our platform uses physiologically relevant nanonets as ultrasensitive force (~nanoNewtons) probes for diagnostic and drug efficacy measurements in disease models at the single and multi-cell resolution.