Fused Fiber Nanonet Based Single and Multi-Cell Diagnostic and Drug Testing Platform

Description:

The role of physical forces in disease onset and progression is widely accepted and this knowledge presents an alternative route to investigating disease models.  Cells both diseased and non-diseased have differences in force signatures.  Measuring forces in real time allows performing diagnostics of cells from patients and also provides a clear path to test drug efficacy.  Recently, numerous force measurement techniques have been developed to probe single and multi-cell behavior.  While these methods have yielded fundamental insights, they are yet unable to capture the fibrous extra-cellular matrix biophysical interactions, involving parameters of curvature, structural stiffness (N m-1), alignment and hierarchy, which have been shown by us to play key roles in disease and developmental biology. The platform technology presented here quantifies high spatio-temporal cell force modulation (both inside-out and outside-in) with and without the presence of a cytoskeleton altering drug using suspended and aligned fiber networks (nanonets) manufactured using the nonelectrospinning Spinnerer base Tunable Engineering Parameters (STEP) technique.  Our platform uses physiologically relevant nanonets as ultrasensitive force (~nanoNewtons) probes for diagnostic and drug efficacy measurements in disease models at the single and multi-cell resolution.

Patent Information:
For Information, Contact:
Grant Brewer
Senior Licensing Associate
Virginia Tech Intellectual Properties, Inc.
(540) 443-9218
gbrewer@vtip.org
Inventors:
Amrinder Nain
Bahareh Behkam
Keywords:
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